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Currently, there are many companies that developp or sell cardiomyocytes derived from human induced pluripotent stem cells. Those cells are mature and need to be freezed or thawed to be conserved if there are not used instantly.
Protocols allowing to recover and seed cardiomyocytes that are freezed lack efficiency. Indeed, survival rate is low and the purity of cells is not high.
A research team from Sorbonne Université has developed a simple protocol allowing the recovery, the seeding and the conservation of cardiomyocytes derived from human induced pluripotent stem cells.
All the ingredients used are commercially available.
• Recovered and seed cardiomyocytes
Differentiated cardiomyocytes were recoverded, at a specific time validated experimentally with the following recovery medium in order to enhance the recovery step exhibiting a purity of the cells close to 95%.
After recovering step, cells can be seeded on any support with cell attachment substrate and begin to contract 1 or 2 days after the seeding.
• Very simple method
• Better purity of cardiomyocytes compared to protocol described in the littérature (95% VS 83%)
• High improvement of the survival rate, specially after freezing / thawing